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mouse nucleus pulposus cells (mnpcs) cp-m146  (Procell Inc)

 
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    Procell Inc mouse nucleus pulposus cells (mnpcs) cp-m146
    Impact of SPP1-positive macrophages on the function of <t>msi-mNPCs.</t> Note: ( A ) Correlation analysis of SPP1 and Macrophage abundance in RNA-seq data; ( B ) Western blot detection of SPP1 expression in Control and msi-IDD mouse IVD tissues; ( C ) Influence of co-culture with oe-NC and oe-SPP1 on the proliferation capacity of msi-mNPCs; ( D ) Influence of co-culture with oe-NC and oe-SPP1 on the apoptosis level of msi-mNPCs, with representative images on the left and quantification on the right; ( E – G ) Effect of oe-NC and oe-SPP1 on the secretion of extracellular matrix and inflammatory factors in msi-mNPCs detected by ELISA; ( H - I ) Influence of co-culture with sh-NC and sh-SPP1 on the proliferation and apoptosis of msi-mNPCs; ( J - L ) Effect of sh-NC and sh-SPP1 on the secretion of extracellular matrix and inflammatory factors in msi-mNPCs detected by ELISA; ( M – N ) Western blot detection of the impact of oe-SPP1 or sh-SPP1 on the expression of msi-mNPC-related factors; All cell experiments were repeated three times; ns P > 0.05, ** P < 0.01, *** P < 0.001
    Mouse Nucleus Pulposus Cells (Mnpcs) Cp M146, supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+nucleus+pulposus+cells+%28mnpcs%29+cp-m146/pmc11748470-83-1-10?v=Procell+Inc
    Average 90 stars, based on 1 article reviews
    mouse nucleus pulposus cells (mnpcs) cp-m146 - by Bioz Stars, 2026-08
    90/100 stars

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    1) Product Images from "Deciphering SPP1-related macrophage signaling in the pathogenesis of intervertebral disc degeneration"

    Article Title: Deciphering SPP1-related macrophage signaling in the pathogenesis of intervertebral disc degeneration

    Journal: Cell Biology and Toxicology

    doi: 10.1007/s10565-024-09948-4

    Impact of SPP1-positive macrophages on the function of msi-mNPCs. Note: ( A ) Correlation analysis of SPP1 and Macrophage abundance in RNA-seq data; ( B ) Western blot detection of SPP1 expression in Control and msi-IDD mouse IVD tissues; ( C ) Influence of co-culture with oe-NC and oe-SPP1 on the proliferation capacity of msi-mNPCs; ( D ) Influence of co-culture with oe-NC and oe-SPP1 on the apoptosis level of msi-mNPCs, with representative images on the left and quantification on the right; ( E – G ) Effect of oe-NC and oe-SPP1 on the secretion of extracellular matrix and inflammatory factors in msi-mNPCs detected by ELISA; ( H - I ) Influence of co-culture with sh-NC and sh-SPP1 on the proliferation and apoptosis of msi-mNPCs; ( J - L ) Effect of sh-NC and sh-SPP1 on the secretion of extracellular matrix and inflammatory factors in msi-mNPCs detected by ELISA; ( M – N ) Western blot detection of the impact of oe-SPP1 or sh-SPP1 on the expression of msi-mNPC-related factors; All cell experiments were repeated three times; ns P > 0.05, ** P < 0.01, *** P < 0.001
    Figure Legend Snippet: Impact of SPP1-positive macrophages on the function of msi-mNPCs. Note: ( A ) Correlation analysis of SPP1 and Macrophage abundance in RNA-seq data; ( B ) Western blot detection of SPP1 expression in Control and msi-IDD mouse IVD tissues; ( C ) Influence of co-culture with oe-NC and oe-SPP1 on the proliferation capacity of msi-mNPCs; ( D ) Influence of co-culture with oe-NC and oe-SPP1 on the apoptosis level of msi-mNPCs, with representative images on the left and quantification on the right; ( E – G ) Effect of oe-NC and oe-SPP1 on the secretion of extracellular matrix and inflammatory factors in msi-mNPCs detected by ELISA; ( H - I ) Influence of co-culture with sh-NC and sh-SPP1 on the proliferation and apoptosis of msi-mNPCs; ( J - L ) Effect of sh-NC and sh-SPP1 on the secretion of extracellular matrix and inflammatory factors in msi-mNPCs detected by ELISA; ( M – N ) Western blot detection of the impact of oe-SPP1 or sh-SPP1 on the expression of msi-mNPC-related factors; All cell experiments were repeated three times; ns P > 0.05, ** P < 0.01, *** P < 0.001

    Techniques Used: RNA Sequencing, Western Blot, Expressing, Control, Co-Culture Assay, Enzyme-linked Immunosorbent Assay

    Impact of the SPP1/PERK/ATF4/IL-10 signaling axis on msi-mNPCs functionality. Note: ( A ) Effects of GSK2656157 treatment on the expression and activation of sh-SPP1-related factors. ( B ) Effect of GSK2656157-treated sh-SPP1 on msi-mNPCs proliferation detected by CCK8 assay. ( C ) Effect of GSK2656157-treated sh-SPP1 on the secretion of Collagen Iα1 and Collagen II in msi-mNPCs detected by ELISA. ( D ) Effect of ATF4 knockdown on sh-SPP1-induced msi-mNPCs proliferation. ( E – F ) Effects of ATF4 knockdown sh-SPP1 on msi-mNPCs proliferation and the secretions of Collagen Iα1 and Collagen II. ( G ) Effects of anti-IL-10 treatment on the expression and activation of sh-SPP1-related factors. ( H - I ) Effects of anti-IL-10 treatment on msi-mNPCs proliferation and the secretions of Collagen Iα1 and Collagen II. All cell experiments were repeated three times. ns P > 0.05, * P < 0.05, ** P < 0.01, *** P < 0.001
    Figure Legend Snippet: Impact of the SPP1/PERK/ATF4/IL-10 signaling axis on msi-mNPCs functionality. Note: ( A ) Effects of GSK2656157 treatment on the expression and activation of sh-SPP1-related factors. ( B ) Effect of GSK2656157-treated sh-SPP1 on msi-mNPCs proliferation detected by CCK8 assay. ( C ) Effect of GSK2656157-treated sh-SPP1 on the secretion of Collagen Iα1 and Collagen II in msi-mNPCs detected by ELISA. ( D ) Effect of ATF4 knockdown on sh-SPP1-induced msi-mNPCs proliferation. ( E – F ) Effects of ATF4 knockdown sh-SPP1 on msi-mNPCs proliferation and the secretions of Collagen Iα1 and Collagen II. ( G ) Effects of anti-IL-10 treatment on the expression and activation of sh-SPP1-related factors. ( H - I ) Effects of anti-IL-10 treatment on msi-mNPCs proliferation and the secretions of Collagen Iα1 and Collagen II. All cell experiments were repeated three times. ns P > 0.05, * P < 0.05, ** P < 0.01, *** P < 0.001

    Techniques Used: Expressing, Activation Assay, CCK-8 Assay, Enzyme-linked Immunosorbent Assay, Knockdown



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    Impact of SPP1-positive macrophages on the function of <t>msi-mNPCs.</t> Note: ( A ) Correlation analysis of SPP1 and Macrophage abundance in RNA-seq data; ( B ) Western blot detection of SPP1 expression in Control and msi-IDD mouse IVD tissues; ( C ) Influence of co-culture with oe-NC and oe-SPP1 on the proliferation capacity of msi-mNPCs; ( D ) Influence of co-culture with oe-NC and oe-SPP1 on the apoptosis level of msi-mNPCs, with representative images on the left and quantification on the right; ( E – G ) Effect of oe-NC and oe-SPP1 on the secretion of extracellular matrix and inflammatory factors in msi-mNPCs detected by ELISA; ( H - I ) Influence of co-culture with sh-NC and sh-SPP1 on the proliferation and apoptosis of msi-mNPCs; ( J - L ) Effect of sh-NC and sh-SPP1 on the secretion of extracellular matrix and inflammatory factors in msi-mNPCs detected by ELISA; ( M – N ) Western blot detection of the impact of oe-SPP1 or sh-SPP1 on the expression of msi-mNPC-related factors; All cell experiments were repeated three times; ns P > 0.05, ** P < 0.01, *** P < 0.001
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    Impact of SPP1-positive macrophages on the function of <t>msi-mNPCs.</t> Note: ( A ) Correlation analysis of SPP1 and Macrophage abundance in RNA-seq data; ( B ) Western blot detection of SPP1 expression in Control and msi-IDD mouse IVD tissues; ( C ) Influence of co-culture with oe-NC and oe-SPP1 on the proliferation capacity of msi-mNPCs; ( D ) Influence of co-culture with oe-NC and oe-SPP1 on the apoptosis level of msi-mNPCs, with representative images on the left and quantification on the right; ( E – G ) Effect of oe-NC and oe-SPP1 on the secretion of extracellular matrix and inflammatory factors in msi-mNPCs detected by ELISA; ( H - I ) Influence of co-culture with sh-NC and sh-SPP1 on the proliferation and apoptosis of msi-mNPCs; ( J - L ) Effect of sh-NC and sh-SPP1 on the secretion of extracellular matrix and inflammatory factors in msi-mNPCs detected by ELISA; ( M – N ) Western blot detection of the impact of oe-SPP1 or sh-SPP1 on the expression of msi-mNPC-related factors; All cell experiments were repeated three times; ns P > 0.05, ** P < 0.01, *** P < 0.001
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    Average 90 stars, based on 1 article reviews
    mouse nucleus pulposus cells mnpcs cp-m146 - by Bioz Stars, 2026-08
    90/100 stars
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    Impact of SPP1-positive macrophages on the function of msi-mNPCs. Note: ( A ) Correlation analysis of SPP1 and Macrophage abundance in RNA-seq data; ( B ) Western blot detection of SPP1 expression in Control and msi-IDD mouse IVD tissues; ( C ) Influence of co-culture with oe-NC and oe-SPP1 on the proliferation capacity of msi-mNPCs; ( D ) Influence of co-culture with oe-NC and oe-SPP1 on the apoptosis level of msi-mNPCs, with representative images on the left and quantification on the right; ( E – G ) Effect of oe-NC and oe-SPP1 on the secretion of extracellular matrix and inflammatory factors in msi-mNPCs detected by ELISA; ( H - I ) Influence of co-culture with sh-NC and sh-SPP1 on the proliferation and apoptosis of msi-mNPCs; ( J - L ) Effect of sh-NC and sh-SPP1 on the secretion of extracellular matrix and inflammatory factors in msi-mNPCs detected by ELISA; ( M – N ) Western blot detection of the impact of oe-SPP1 or sh-SPP1 on the expression of msi-mNPC-related factors; All cell experiments were repeated three times; ns P > 0.05, ** P < 0.01, *** P < 0.001

    Journal: Cell Biology and Toxicology

    Article Title: Deciphering SPP1-related macrophage signaling in the pathogenesis of intervertebral disc degeneration

    doi: 10.1007/s10565-024-09948-4

    Figure Lengend Snippet: Impact of SPP1-positive macrophages on the function of msi-mNPCs. Note: ( A ) Correlation analysis of SPP1 and Macrophage abundance in RNA-seq data; ( B ) Western blot detection of SPP1 expression in Control and msi-IDD mouse IVD tissues; ( C ) Influence of co-culture with oe-NC and oe-SPP1 on the proliferation capacity of msi-mNPCs; ( D ) Influence of co-culture with oe-NC and oe-SPP1 on the apoptosis level of msi-mNPCs, with representative images on the left and quantification on the right; ( E – G ) Effect of oe-NC and oe-SPP1 on the secretion of extracellular matrix and inflammatory factors in msi-mNPCs detected by ELISA; ( H - I ) Influence of co-culture with sh-NC and sh-SPP1 on the proliferation and apoptosis of msi-mNPCs; ( J - L ) Effect of sh-NC and sh-SPP1 on the secretion of extracellular matrix and inflammatory factors in msi-mNPCs detected by ELISA; ( M – N ) Western blot detection of the impact of oe-SPP1 or sh-SPP1 on the expression of msi-mNPC-related factors; All cell experiments were repeated three times; ns P > 0.05, ** P < 0.01, *** P < 0.001

    Article Snippet: The mouse nucleus pulposus cells (mNPCs) (CP-M146, China, provided by Procell) were cultured in Thermo Fisher's DMEM/F-12 (11320033, USA).

    Techniques: RNA Sequencing, Western Blot, Expressing, Control, Co-Culture Assay, Enzyme-linked Immunosorbent Assay

    Impact of the SPP1/PERK/ATF4/IL-10 signaling axis on msi-mNPCs functionality. Note: ( A ) Effects of GSK2656157 treatment on the expression and activation of sh-SPP1-related factors. ( B ) Effect of GSK2656157-treated sh-SPP1 on msi-mNPCs proliferation detected by CCK8 assay. ( C ) Effect of GSK2656157-treated sh-SPP1 on the secretion of Collagen Iα1 and Collagen II in msi-mNPCs detected by ELISA. ( D ) Effect of ATF4 knockdown on sh-SPP1-induced msi-mNPCs proliferation. ( E – F ) Effects of ATF4 knockdown sh-SPP1 on msi-mNPCs proliferation and the secretions of Collagen Iα1 and Collagen II. ( G ) Effects of anti-IL-10 treatment on the expression and activation of sh-SPP1-related factors. ( H - I ) Effects of anti-IL-10 treatment on msi-mNPCs proliferation and the secretions of Collagen Iα1 and Collagen II. All cell experiments were repeated three times. ns P > 0.05, * P < 0.05, ** P < 0.01, *** P < 0.001

    Journal: Cell Biology and Toxicology

    Article Title: Deciphering SPP1-related macrophage signaling in the pathogenesis of intervertebral disc degeneration

    doi: 10.1007/s10565-024-09948-4

    Figure Lengend Snippet: Impact of the SPP1/PERK/ATF4/IL-10 signaling axis on msi-mNPCs functionality. Note: ( A ) Effects of GSK2656157 treatment on the expression and activation of sh-SPP1-related factors. ( B ) Effect of GSK2656157-treated sh-SPP1 on msi-mNPCs proliferation detected by CCK8 assay. ( C ) Effect of GSK2656157-treated sh-SPP1 on the secretion of Collagen Iα1 and Collagen II in msi-mNPCs detected by ELISA. ( D ) Effect of ATF4 knockdown on sh-SPP1-induced msi-mNPCs proliferation. ( E – F ) Effects of ATF4 knockdown sh-SPP1 on msi-mNPCs proliferation and the secretions of Collagen Iα1 and Collagen II. ( G ) Effects of anti-IL-10 treatment on the expression and activation of sh-SPP1-related factors. ( H - I ) Effects of anti-IL-10 treatment on msi-mNPCs proliferation and the secretions of Collagen Iα1 and Collagen II. All cell experiments were repeated three times. ns P > 0.05, * P < 0.05, ** P < 0.01, *** P < 0.001

    Article Snippet: The mouse nucleus pulposus cells (mNPCs) (CP-M146, China, provided by Procell) were cultured in Thermo Fisher's DMEM/F-12 (11320033, USA).

    Techniques: Expressing, Activation Assay, CCK-8 Assay, Enzyme-linked Immunosorbent Assay, Knockdown